![]() ![]() This was corroborated by the observation that over 90 % occurred within homopolymer regions of greater than 4 nucleotides in length. These variations were confirmed by Sanger sequencing and subsequent analysis suggested that the discrepancies were sequencing errors in the published genome not true single nucleotide polymorphisms. ![]() We performed next generation sequencing using Illumina MiSeq technology on the DSM10061 strain of Clostridium autoethanogenum and observed 243 single nucleotide discrepancies when compared to the published finished sequence (NCBI: GCA_000484505.1), with 59.1 % present in coding regions. However, crucial to metabolic modelling and directed pathway engineering is a reliable and comprehensively annotated genome sequence. Current research efforts are focused on the enhancement and extension of product formation in this organism via synthetic biology approaches. This common industrial waste gas can act as the sole energy and carbon source for the bacterium that converts the low value gaseous components into cellular building blocks and industrially relevant products via the action of the reductive acetyl-CoA (Wood-Ljungdahl) pathway. Clostridium autoethanogenum is an acetogenic bacterium capable of producing high value commodity chemicals and biofuels from the C1 gases present in synthesis gas.
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